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BIOEXTREMDEGRAD

Sobre o projeto

Existe um grande potencial biotecnológico em comunidades bacterianas de ambientes extremos, onde prevalecem e proliferam estirpes com mecanismos que permitem uma adaptação vantajosa a esses ambientes. De fato, a bioaumentação de estirpes de bactérias com capacidades metabólicas de interesse em reatores biológicos para tratamento de águas residuais é um conceito conhecido que tem sido estudado em sistemas convencionais de ETAR com lamas floculares. Além disso, a adaptação dos microrganismos a ambientes extremos pode depender da sua capacidade de usar moléculas recalcitrantes como fonte de carbono, nitrogênio e energia, e essa capacidade às vezes depende de enzimas catabólicas codificadas por genes que estão em plasmídeos. É também reconhecido que a transferência conjugativa de plasmídeos pode ser explorada para aumentar as capacidades de biodegradação em biorreatores. Este projeto visa aplicar estratégias inovadoras para desenvolver e otimizar processos contribuindo para o objetivo de alcançar a degradação total dos poluentes organicos emergentes em sistemas de ETAR com lamas granulares aeróbias.

Para mais informações sobre os objetivos e métodosdo projeto consulte este documento, para informações sobre os resultados do projeto consulte este documento.

bacterial strain TIBU

Bioaumentação e transferência de plasmídeos por conjugação utilizando bactérias de ambientes extremos para aumentar a biodegradação de poluentes recalcitrantes em lama granular de ETAR

Datas
-
Coordenação do CCMAR
Jorge Daniel Dias Carlier
Referência
PTDC/CTA-AMB/7782/2020
Agencias de financiamento
FCT - Fundação para a Ciência e Tecnologia
Orçamento Total
213629.00 €
Orçamento do CCMAR
213629.00€

Atividades

Objetivo Geral

Desenvolver e otimizar processos baseados em estratégias de bioaumentação para alcançar a biodegradação completa de fármacos em sistemas de estações de tratamento de águas residuais (ETAR) com lamas granulares aeróbias.

Metas

Fármacos Modelo: Inicialmente, foram selecionados o paracetamol, o ibuprofeno e a fluoxetina, devido ao seu uso generalizado e frequente detecção no ambiente.

Fármacos Adicionais: Posteriormente, incluíram-se a carbamazepina e o diclofenac, reconhecidos pela sua elevada persistência nos sistemas de ETAR.

Fármaco de Impacto Ecológico: O 17α-etinilestradiol foi também incorporado, dado o seu potencial impacto significativo nos ecossistemas.

Microplásticos Estudados: Foram analisados filmes provenientes de uma garrafa de polietileno tereftalato (PET) e de um saco de polietileno compostável (CPE) como microplásticos alvo.

Tarefas do Projeto

T1 – Seleção de estirpes bacterianas com capacidade para degradar os fármacos alvo.

T2 – Identificação de estirpes degradativas que possuam plasmídeos com genes envolvidos em vias catabólicas.

T3 – Início e operação de reatores para a formação de grânulos microbianos aeróbios semi-maturados.

T4 – Cultivo de grânulos microbianos aeróbios maduros, com e sem bioaumentação.

T5 – Avaliação da biodegradação dos poluentes pelos grânulos bioaumentados e não bioaumentados.

Publicações

Peer-Reviewed International Papers in periodic scientific journals: 

  • Lara-Moreno A, Aguilar-Romero I, Madrid F, Villaverde J, Carlier JD, Santos JL, Alonso E, Morillo E (in preparation) Novel bacterial strains and bacterial consortia for nonylphenol removal in water and sewage sludge. Insights from gene expression and toxicity reduction.
  • Adriana González Alcivar, Jorge D. Carlier, Maria Clara S. Costa and Isabel Marín Beltrán (in preparation) Compostable and conventional microplastics suffer minor changes under simulated composting conditions. 
  • Carlier JD, Igbodo B, Lara-Moreno A, Costa MC (submitted) Studies on the bioaugmentation of Mycolicibacterium aubagnense HPB1.1 in aerobic granular sludge from a WWTP: adaptability of native prokaryotes and enhancement of paracetamol intermediate metabolites biodegradation. Submitted to Environmental Technology & Innovation.
  • Lara-Moreno A, Vargas-Ordóñez A, Villaverde J, Madrid F, Carlier JD, Santos JL, Alonso E, Morillo E (2024) Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway. Journal of Hazardous Materials. 136128.
  • Lara-Moreno A, Costa MC, Vargas-Villagomez A, Carlier JD (2024) New bacterial strains for ibuprofen biodegradation: Drug removal, transformation, and potential catabolic genes. Environmental Microbiology Reports.
  • Lara-Moreno A, El-S. Ismail F, Cox CJ, Costa MC, Carlier JD (2024) Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes. Applied Water Science 14, 198
  • Palma TL, Costa MC (2024) Biodegradation of 17α-Ethinylestradiol by Strains of Aeromonas Genus Isolated from Acid Mine Drainage. Clean Tecnologies. 6, 116–139.
  • Carlier JD, dos Reis Ferreira GM, Schwan RF, da Silva CF, Costa MC (2024) Pb2+ biosorption by Serratia marcescens CCMA 1010 and relation of zntR gene expression with these ions’ efflux regulation. Environmental Advances 15, 100479.
  • Palma T, Valentine J, Gomes V, Faleiro M, Costa M. Batch Studies on the Biodegradation Potential of Paracetamol, Fluoxetine and 17α-Ethinylestradiol by the Micrococcus yunnanensis Strain TJPT4 Recovered from Marine Organisms. Water. 2022; 14(21):3365.

 

Peer-Reviewed International Papers in proceedings of international congresses:

  • Carlier JD, Lara-Moreno A, Ngonyani AM, Martins A, V Villagomez AV, Igbodo B, Cox C, El-S Ismail F, Marín-Beltrán I, Palma TL, Costa MC (2023) Selection of bacterial strains to bioaugment granular sludge and improve the removal of recalcitrant pollutants. 18th International Conference on Environmental Science and Technology CEST2023. 30 August - 2 September. Athens, Greece. https://doi.org/10.30955/gnc2023.00371  

 

Genomic sequences of bacterial strains published in public databases:

  • Klebsiella pneumoniae TIBU2.1 bacterial strain complete genome uploaded at NCBI: Accession numbers CP139935 and CP139936.     https://www.ncbi.nlm.nih.gov/nuccore/?term=Carlier%20Klebsiella%20TIBU2.1  
  • Stutzerimonas stutzeri CSW02 bacterial strain complete genome uploaded at NCBI: Accession numbers CP140616 to CP140618.     https://www.ncbi.nlm.nih.gov/nuccore/?term=Carlier%20Stutzerimonas%20CSW02  
  • Mycolicibacterium aubagnense HPB1.1 bacterial strain complete genome uploaded at NCBI: Accession numbers CP122994 to CP12299.     https://www.ncbi.nlm.nih.gov/nuccore/?term=Mycolicibacterium+HPB1.1+Carlier  
  • Micrococcus yunnanensis strain TJPT4 bacterial strain complete genome uploaded at NCBI: Accession number CP125290.     https://www.ncbi.nlm.nih.gov/nuccore/?term=Micrococcus+yunnanensis+TJPT4+Carlier  

 

Bacterial strains in collections:

  • Mycolicibacterium aubagnense HPB1.1 - accepted in the open collection of the Agricultural Research Service (ARS) Culture Collection (NRRL) in the USA) – accession code assigned: B-65706
  • Klebsiella pneumoniae TIBU2.1 - accepted in the open collection of the Agricultural Research Service (ARS) Culture Collection (NRRL) in the USA) – accession code assigned: B-65705
  • Micrococcus yunnanensis TJPT4 - accepted in the open collection of the Agricultural Research Service (ARS) Culture Collection (NRRL) in the USA – accession code assigned: B-65704

 

Oral presentations at international congresses:

  • Gonzalez-Alcivar A, Carlier JD, Costa MC, Marín-Beltrán I (2024) MP biodegradation in sewage sludge subjected to composting conditions (“Biodegradación de microplásticos en lodos de depuradora en condiciones de compost”). 3ª JORNADAS SOBRE CONTAMINACIÓN POR PLÁSTICOS. 15 - 16 October 2024 in Barcelona, Spain.
  • Lara-Moreno A, Madrid F, Morillo E, Vargas-Ordóñez A, Aguilar-Romero I, Carlier J, Villaverde J (2024). Application of Novel Bacterial Strains for Paracetamol Mineralization in Aqueous and Sewage Sludge Systems. 34th annual meeting of the Society of Environmental Toxicology and Chemistry – Europe (SETAC Europe), 5–9 May 2024. Seville, Spain.
  • Lara-Moreno A, Costa MC, El-Sayed F, Igbodo B, Carlier JD (2023) Selection of paracetamol and its intermediates metabolites degrading bacteria for bioaugmentation of granular sludge. 6th International Symposium on Water Pollution and Treatment (ISWPT 2023). 14 and 15 October. Bangkok, Thailand.
  • Palma T, Valentine J, Lara-Moreno A, Costa MC, Carlier JD (2023) Bacteria enriched from marine organisms with potential for the removal of some pharmaceutical compounds. 1st European GREEN Conference - EGC 2023. 23-26 May. Vodice, Croacia
  • Carlier JD, Lara-Moreno A, Ngonyani AM, Martins A, V Villagomez AV, Igbodo B, Cox C, El-S Ismail F, Marín-Beltrán I, Palma TL, Costa MC (2023) Selection of bacterial strains to bioaugment granular sludge and improve the removal of recalcitrant pollutants. 2. 30 August - 2 September. Athens, Greece
  • Gonzalez-Alcivar A, Marín-Beltrán I (2023) Biodegradación de microplásticos compostables y convencionales por comunidades bacterianas de lodos de depuradora” XII evento internacional La Universidade en el siglo XXI - VI Congreso Internacional - Industria y Desarrollo Sostenible (CIIDS). Escuela Superior Politécnica Agropecuaria de Manabí Manuel Félix López, Equador, 16 – 17 November 2023.

 

Presentations at national congresses:

  • Anja Udundzic, Jorge Dias Carlier, Alba Lara Moreno, Maria Clara Costa (2024) Identification of Bacterial Strains Competent in Biodegrading Carbamazepine, Diclofenac, and 17-α-Ethinylestradiol–Preliminary results. 15th National Organic Chemistry Meeting (15ENQO), and the 8th National Medicinal Chemistry Meeting (8ENQT), in Faro, Portugal 22 – 24 January 2024
  • Alcivar AG, Marín-Beltrán I, Carlier JD, Costa MC (2023) Effect of composting from sewage sludge on microplastic biodegradation. Symposium of the Erasmus Mundus MSc in Chemical Innovation and Regulation (ChIR). 3-4th July, Faro, Portugal.  

 

Other communications:  

  • The BIOEXTREMDEGRAD Project - a one-hour lecture by Dr. Jorge D. Carlier, as a part of the Seminars in Metagenomics unit of the Master course program in Molecular and Microbial Biology at the University of Algarve, on October 24, 2024 (by invitation of Professor Maria Leonor Faleiro, who is responsible for the course unit mentioned).

 

Master's thesis framed in the project:  

  • Anja Udundzic. Searching for bacteria degrading carbamazepine, diclofenac sodium, and 17α-ethinylestradiol from sewage sludge digestor sample and in silico identification of genes potentially involved in the biodegradation of these drugs. Thesis for the title Master of Science (MSc) in the Erasmus Mundus master in Quality in Analytical Laboratories. University of Algarve. September 25, 2024.  
  • Ayleen Villagomez. Biodegradation of ibuprofen and fluoxetine by bacterial strains isolated from environmental samples and identification of candidate catabolic genes. Thesis for the title Master of Science (MSc) in Biotechnology. University of Algarve. December 7, 2023. http://hdl.handle.net/10400.1/20756  
  • Adriana Rosaura Gonzalez Alcivar. Effect of composting on biodegradation of compostable and conventional microplastic by bacterial communities from sewage sludge. Thesis for the title Master of Science (MSc) on the Erasmus Mundus master Chemical Innovation and Regulation. University of Algarve. September 21, 2023. http://hdl.handle.net/10400.1/25717  
  • Benjamin Igbodo. Implementation of standard methods for parameters routinely monitored in wastewater treatment plants and application in bioaugmentation with Mycolicibacterium sp. In granular sludge reactors. Thesis for the title Master of Science (MSc) on the Erasmus Mundus Master in Quality in Analytical Laboratories. University of Algarve. August 15, 2023. http://hdl.handle.net/10400.1/25662  
  • Alois Mathew Ngonyani. Marine bacteria and their role in polyethylene terephthalate biodeterioration and biofragmentation. Thesis for the title Master of Science (MSc) on the Erasmus Mundus Master in Quality in Analytical Laboratories. University of Algarve. January 20, 2023. https://sapientia.ualg.pt/handle/10400.1/19609  
  • Fatma Mahmoud Ahmed El-Sayed Ismail. entitled “Biodegradation of paracetamol and its metabolite hydroquinone by bacterial strains isolated from two mine of the Iberian Pyrite Belt. Thesis for the title Master of Science (MSc) on the Erasmus Mundus Master in “Quality in Analytical Laboratories. University of Algarve. December 12, 2022. https://sapientia.ualg.pt/handle/10400.1/19613  

Coordenação do CCMAR

Financiado por

FCT - Fundação para a Ciência e Tecnologia

Operador de programa

CRESC Algarve 2020
COMPETE2020
EMBRC