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BIOEXTREMDEGRAD

Project Summary

Wastewater treatment plants (WWTPs) are the primary source of pharmaceutical drugs and the metabolic products of their degradation to the environment. Indeed, concerns about harmful environmental impact are growing due to chronic trace concentration exposure caused by WWTPs’ inability to completely degrade pharmaceuticals. In addition, WWTPs have also been reported to be a source of microplastics to the aquatic environments, especially of microfibers released from washing clothes. It is known that there is a great biotechnological potential in bacterial communities from extreme environments, where strains with mechanisms allowing advantageous adaptation to these environments can prevail and proliferate. Moreover, the adaptation of microorganisms to extreme environments, in some cases, depends on their ability to use recalcitrant molecules as a source of carbon, nitrogen, and energy. That ability might depend on catabolic enzymes encoded by genes that may be present in plasmids. It is also recognized that conjugative plasmid transfer can be exploited to enhance biodegradation capabilities in bioreactors. This project aimed to apply bioaugmentation strategies to develop and optimize processes to improve the biodegradation of emerging pollutants in WWTP systems with aerobic granular sludge.​

For more information on project goals and methods refer to this document, and for more information on project results refer to this document.

bacterial strain TIBU

Bioaugmentation and conjugative plasmid transference using bacteria from extreme environments to enhance biodegradation of recalcitrant pollutants in WWTP granular sludge

Start and End Date
-
CCMAR Coordinators
Jorge Daniel Dias Carlier
Project reference
PTDC/CTA-AMB/7782/2020

Funding agencies

FCT - Fundação para a Ciência e Tecnologia
Total Budget
213629.00 €
CCMAR Budget
213629.00€

Project Activities

General Goal

Develop and optimize processes based on Bioaugmentation strategies to achieve total biodegradation of pharmaceuticals in WWTPs systems with aerobic granular sludge. ​

Targets

  • Paracetamol, Ibuprofen, and Fluoxetine were the pharmaceuticals planned to be used as models because they are among the most used, and are frequently  detected
  •  Carbamazepine and Diclofenac were later included because they are highly persistent in WWTP systems
  • 17α-ethinylestradiol was finally also included since it can have a high impact on ecosystems
  • Films from a polyethylene terephthalate (PET) bottle and a bag of compostable polyethylene (CPE) were the target microplastics (MP) studied

Project Tasks

T1 – Selection of bacterial strains with a role in the degradation of target drugs​

T2 – Selection of degradative strains carrying plasmids with genes involved in catabolic pathways​

T3 – Reactors start-up and operation to form half-maturated aerobic microbial granules​

T4 – Cultivation of bioaugmented and non-bioaugmented mature aerobic microbial granules

T5 – Biodegradation of pollutants by bioaugmented and non-bioaugmented granules

Publications

  • Carlier JD, Igbodo B, Lara-Moreno A, Costa MC (submitted) Studies on the bioaugmentation of Mycolicibacterium aubagnense HPB1.1 in aerobic granular sludge from a WWTP: adaptability of native prokaryotes and enhancement of paracetamol intermediate metabolites biodegradation. Submitted to Environmental Technology & Innovation.
  • Lara-Moreno A, Vargas-Ordóñez A, Villaverde J, Madrid F, Carlier JD, Santos JL, Alonso E, Morillo E (2024) Bacterial bioaugmentation for paracetamol removal from water and sewage sludge. Genomic approaches to elucidate biodegradation pathway. Journal of Hazardous Materials. 136128.
  • Lara-Moreno A, Costa MC, Vargas-Villagomez A, Carlier JD (2024) New bacterial strains for ibuprofen biodegradation: Drug removal, transformation, and potential catabolic genes. Environmental Microbiology Reports.
  • Lara-Moreno A, El-S. Ismail F, Cox CJ, Costa MC, Carlier JD (2024) Batch studies on the biodegradation of paracetamol and 1,4-hydroquinone by novel bacterial strains isolated from extreme environmental samples and the identification of candidate catabolic genes. Applied Water Science 14, 198
  • Palma TL, Costa MC (2024) Biodegradation of 17α-Ethinylestradiol by Strains of Aeromonas Genus Isolated from Acid Mine Drainage. Clean Tecnologies. 6, 116–139.
  • Carlier JD, dos Reis Ferreira GM, Schwan RF, da Silva CF, Costa MC (2024) Pb2+ biosorption by Serratia marcescens CCMA 1010 and relation of zntR gene expression with these ions’ efflux regulation. Environmental Advances 15, 100479.
  • Palma T, Valentine J, Gomes V, Faleiro M, Costa M. Batch Studies on the Biodegradation Potential of Paracetamol, Fluoxetine and 17α-Ethinylestradiol by the Micrococcus yunnanensis Strain TJPT4 Recovered from Marine Organisms. Water. 2022; 14(21):3365.

CCMAR Coordinators

Funded by

FCT - Fundação para a Ciência e Tecnologia

Programme Operator

CRESC Algarve 2020
COMPETE2020
EMBRC