Principles and Aplications of Real Time PCR systems

CCMAR has acquired two Real-Time PCR systems"CFX Touch" to reinforce the capabilities of the Molecular Biology Platform and the scientific community in general. The CFX system is a Real-Time PCR system based on the C1000 Touch thermal cycler. The CFX block is capable of generating a thermal gradient to optimize the PCR experiments. The System is compatible with the fluorescent techniques used in PCR amplification. The software CFX Maestro allows the acquisition, visualization and analysis of the data generated by the CFX, and supports applications such as quantification, melting curve analysis, end point analysis, allelic discrimination and analysis of gene expression and ANOVA tests.