Date: 8 Julho
Time: 11:15 - 12:30
Location: Anfiteatro Verde, Edifício 8, Campus de Gambelas
"The potential of on-line UPLC IMS-MS for quantitative and qualitative protein profiling of complex samples"
By Dr Robert Tonge (Waters)
This presentation will give an overview of some of the analytical challenges in shotgun, or 'bottom-up' proteomics, before introducing the LC-MSE data-independent acquisition method and how it is further enhanced by the addition of ion-mobility separations. Performance examples of High Definition Mass Spectrometry (HDMS) will be provided, along with comparisons with the more traditional-type data-dependent acquisition (DDA) strategies. In addition to providing protein identifications, the LC-MSE protocol can also provide fully quantitative data using an accurate and efficient label-free strategy. The presentation will finish by giving updates on the latest Waters hardware releases for proteomics; the Synapt G2-S HDMS-enabled mass spectrometer, and the TRIZAIC microfabricated nanotile which brings significant improvements in ease of use and robustness to nanoscale LC separations.